At present, ion torrent offers the onetimeuse ion 314 sequencing chip figure 4. After the initialization is complete, the chip is loaded onto the instrument. I have loaded a ion torrent 318 chip today,but the loading is very bad. The ion torrent certified service provider program is a global network of validated service providers that enable researchers in small and mediumsized laboratories to get highquality transcriptome sequencing data generated on the ion proton instrument. I have tried reducing my library concentration but it has not worked for me, can anybody suggest a. Next generation sequencing ion torrent semiconductor. The ion torrent personal genome machine pgm is a smallscale. The throughput of the ion torrent 314 and ion torrent 316 v2 chips has increased 10 fold to 100 mb and 1 gb respectively, and the ion torrent 318 chip throughput has doubled to 2 gb. Ion torrent released three more datasets this morning, all generated on their 318 chip.
The subsequent libraries after barcoding and pooling can be run on a 314, 316 or 318 chip after emulsion pcr of the library fragments. Ion pgm and ion proton system chips thermo fisher scientific us. That is you can see a very clear boundary between the two parts. They have also launched their ion onetouch system, which automates much of the process, reducing hands on time to a few minutes per sample. Ion torrent semiconductor sequencers produce an average read length of approximately 400 nucleotides per read. These high quality libraries are prepared rapidly by pcr with as little as 10 ng of ffpe dna or genomic dna. Landscape of nextgeneration sequencing technologies. Read length is expected to increase to 400 bp in 2012. Ion torrent next gen sequencing fast turnaround with semiconductor sequencing. The ion 318 chip v2 bc detects polymerasedriven base incorporation and translates this information into. Recommended configuration 6 sample per ion 318 chip amplicons with target base coverage at 500x. Ion pgm 318 chip, ion pgm 318 chip v2, ion 318 chip textual definition. Torrent suite dx software the ion pgm dx system is used in conjunction with the following kits.
This is a method of sequencing by synthesis, during which a complementary strand is built based on the sequence of a template strand. Back in 20 i used to work at a lab that had an ion torrent proton. With as little as 10 ng input dna, the new ion 314, 316, and 318 chips yield 99. An ion torrent sequencer pgm personal genome machine has been. The ion 318 chip kit v2 bc contains 8 barcoded chips for sample tracking and sequencing runs using the ion personal genome machine pgm system. Includes torrent suite software with all necessary software components to deliver signal processing, base calling, read alignment, and variant calling. Sample preparation and quantification oncomine dx target. Comparison of two highthroughput semiconductor chip. Ion pgm system for nextgeneration sequencing thermo. The ion 316 chip kit v2 bc contains 8 barcoded chips for sample tracking and sequencing runs using the ion personal genome machine pgm system, the first postlight semiconductorbased sequencer. Ion torrent semiconductor nextgeneration sequencing.
I have run some chips 318 on ion torrent but i am getting high polyclonal beads many times. Ion pgm system outperforms competitor on major metrics. The sequencing reaction is performed using the ion pgm dx sequencing kit on the ion pgm dx sequencer, which measures hydrogen ions that are generated during the incorporation of nucleotides into the nascent strand. The quality of pgm sequences has not been reassessed, and results have not been compared in the context of a genetargeted microbial ecology study. The ion pi sequencing 200 kit v2 can be used with up to 200. Ion torrent has increased the number of accessible sensors on the ion 318 chip to 11 million, from 1. Evaluation of the ion torrent personal genome machine for. Can anyone working on iontorrent suggest a good way to. The ion 318 chip kit v2 bc contains 8 barcoded chips for sample tracking and sequencing runs using the ion personal genome machine pgm system, the first. Thermo fisher scientific signs distribution agreement with. The ion 316 chip will produce up to 1 gb throughput, compared to its original 100 mb throughput, and the ion 318 chip will produce as much as 2 gb throughput, compared to its original 1. Using ion ampliseq custom solutions, researchers can. The ion 316 chip v2 bc detects polymerasedriven base incorporation and translates this information into.
In 2012, life technologies introduced a new generation of ion semiconductor sequencers. The chips are different in the number of wells resulting in higher production within the same sequencing time. Create and add a custom barcode set on ion pgm torrent server. Separately, using different samples, ion proton sequencerbased results have also demonstrated successfully that ion semiconductor sequencing is suitable. Huge lot ion torrent sequencing chip solutions reactions. The chip is compatible with the ion torrent pgm and has a run time of. It is confused to see the loading image,the upper half is very dense,the lower half is very sparpe. Barcoded library, gdna or long amplicons, with pippin prep size selection, lib. Elim is known for high quality, fast turnaround times, and costeffective ngs services. The ion 318 chip enables the production of 1 gb data in 2 hours.
The ion 318 chip kit v2 bc contains 8 barcoded chips for sample tracking and sequencing runs using the ion personal genome machine pgm system, the first postlight semiconductorbased sequencer. If the nucleotide is incorporated, a hydrogen ion is released. The sequencing run is started and runs according to the plan prepared before the run. The ion torrent ion chef system simplifies the workflow for ion genestudio s5 series, ion pgm, and ion proton systems, providing an automated, walkaway workflow solution for ion ampliseq library preparation and reproducible template preparation and chip loading. The ion pgm dx instrument system is intended for targeted sequencing of human genomic dna. How many samples can be run at the same time in a 3. Can anyone advise me on poor chip loading density for ion. We provide library preps, enrichment, and bioinformatics as part of our comprehensive ngs services on the ion torrent platform. Ion 318 chip v2 bc you can perform mixed ion chef runs with one ion 316 chip v2 bc and one ion 318 chip v2 bc using the ion pgm hi q view chef kit or the ion pgm hi q view chef 400 kit requires torrent suite software 5. Nonetheless, we routinely run 68 samples per 318 chip for 66000 amplicons, and get 37 miliion q20 bases. Ion semiconductor sequencing is a method of nextgeneration sequencing based on the detection of hydrogen ions that are released during the polymerization of dna.
Evaluation of the ion torrent personal genome machine for gene. Lastly, we used the ion torrent pgm 318 chip with the hiq kit to sequence nifh amplicons from bulk soil samples as a case study to characterize. The ion 314 chip v2 bc detects polymerasedriven base incorporation and translates. The 96barcoded bidirectional library was sequenced through pgm, ion torrent life technologies with the ion 318 chip and ion pgm 400 sequencing kit life technologies, according to.
Ion torrent has even announced the piii chip which will double the number of sensors, generating 200gb per run. The following tables show a highlevel view of the dataflow. Template kit compatibility the ion pi sequencing 200 kit v2 must be used with the ion pi template ot2 200 kit v2 cat. Ion pgm system for nextgeneration sequencing thermo fisher. Ion pgm and ion proton system chips thermo fisher scientific. Ion pi sequencing 200 kit v2 kansas state university. Multiplexing allows multiple samples to be processed simultaneously on the same chip, which reduces costs, time, and. Company profile page for ion torrent systems inc including stock price, company news, press releases, executives, board members, and contact information.
File sizes for torrent data depend on the torrent suite software version, chip type, and kit. Ion 316 chip v2 4488145 ion 318 chip v2 4488146 ion template preparation kits. Life technologies to launch ion 318 chip in second half of. Pgm sequencing perelman school of medicine university of. Ion torrent 318 chips, shirleyhs, ion torrent, 28, 122320 08. Conclusions similar quality between 318 chip and barcoding barcoded and non ion torrent community barcoded runs technical details robust set of barcodes desired features losing first 11 high troubleshooting quality bases to the barcode explains lower initial quality. July 2015 box seal opened, 2x packs inside and they are sealed. Comparison of the performance of ion torrent chips in. The ion 314 chip 1 million wells 10 mb output 316 the ion 316 chip 6 million wells 100 mb output 318 the ion 318 chip 11 million wells 1 gb output the ion pgm sequencer is ideal for sequencing small genomes, sets of genes, or performing gene expression profiling or chipseq. The ion 318 chip v2 is a semiconducting chip manufactured by life technologies which detects polymerasedriven base incorporation to translate into digital form. Ion torrent personal genome machine the chip is the machine. The high ctc purity rate, in conjunction with the ion torrent pgms capability to accurately sequence as little as 10ng of dna, enables highly multiplexed interrogation on one ion 318 sequencing. Chips ion 314 chip v2 4482261 see your sequencing user guide for instructions.
But, with the 318 chip it will approach the weekly throughput assuming 8hour workdays of the venerable gaiix again, with gaiix running unattended. Ion torrent is working to improve empcr, first through the release of the ion xpress kit, which reduced the overall time from 6 hours to 3. From dna extraction to data analysis, within only one day, 64. Recommended application somatic mutation detection. It takes only 2 to 3 days to finish the whole pipeline, from library construction to the sequencing itself. The ion torrent dataflow involves the transfer of raw sequencing data from the ion pgm or ion proton s equencer to the torrent server for analysis and reporting. Ion 314 chip ion 316 chip ion 318 chip basecalls alignment variant calls amplify targets sizeselect cleanup sequencing data analysis. We have stuck with the ion torrent pgmprototon platform through all of the. Recommended application identification of gene variants correlating with antimicrobial resistance in mycobacterium tuberculosis.
We also run 2 whole exome libraries per 318 chip with good results. Thermo fishers ion torrent uses semiconductor sequencing technology. The ion 316 sequencing chip is being released during the second quarter of 2011. Ion torrent offers an ampliseq cancer panel of 50 most common cancer genes with 2,800 cosmic mutations. The ion 314 sequencing chip produces 10 mb of data at greater than 99% raw accuracy table 1. It adopts ion torrent semiconductor sequencing technology for next it adopts ion torrent semiconductor sequencing technology for nextgeneration sequencing, which does not depend on any optical imaging system at all. Singletube preparation of amplicon libraries from genomic hotspot regions that frequently mutate in human cancer genes. Ion torrent would definitely need a 320 chip to really go headtohead with hiseq 2000 on cost. Ion torrent produced panels listed below prices vary by panel. The ion torrent platform can utilize one of the three available ion chips. Lastly, we used the ion torrent pgm 318 chip with the hiq kit to sequence nifh amplicons from bulk soil samples as a case study to characterize the. In that study, only eight maternal plasma dna samples were used, comprising four normal pregnancies and four with trisomy 21 fetuses, which were sequenced on ion torrent 314316318pi chips. Affordable sequencing with ion pgm v2 chips that dramatically reduce cost. The 316 chip contains five times the number of wells but produces more than 100 mb of sequencing data.